Explanation of Data Bases and Bar Graphs

RNA-Seq data: Median TPM for tumor cells (red), macrophages (TAM; blue) and T cells (TAT; green) from ascites (Reinartz et al., 2016; Worzfeld et al., 2018). Grey: TCGA RNA-Seq data (median TPM) for solid tumors, TPM normalized to RNA-Seq data for ascites tumor cells (Peng et al., 2015).

Proteomic data:

LC/MS-MS analysis (median LFQ) of TU, TAM and TAT from ascites (Worzfeld et al., 2018).

 

Secretome data:

LC/MS-MS analysis (median LFQ) of conditioned medium from ascites-derived TU, TAM and TAT (5-hr culture in protein-free medium) (Worzfeld et al., 2018).

SOMAscan affinity proteomics data:

SOMAscan signals reflecting relative levels of protein mediators in ascites, plasma from ovarian cancer patients (OC-plasma) and patients with non-malignant gynecologic diseases (N-plasma)  (Finkernagel et al., 2019).

 

Box pots show medians (horizontal line), upper and lower quartiles (box), range (whiskers) and outliers/fliers (diamonds). 

 

Association of transcriptome and survival data:

  1. RFS associated with TCGA RNA-Seq data.

  2. KM-Plotter relapse-free survival data for high grade serous ovarian cancer (Gyorffy et al., 2012).

  3. PRECOG overall survival data for ovarian cancer (Gentles et al., 2015).

 

TCGA and KMP: data split at best fitting quantile; PRECOG: data split at median.

A z-zcore of >1.96 or <-1.96 corresponds to a logrank p-value of <0.05.

Positive z-scores indicate a hazard ratio >1 (shorter survival); negative z-scores indicate a hazard ratio <1 (longer survival).

OvaRA, University of Marburg, Germany

© 2017 by Rolf Müller