Explanation of Data Bases and Bar Graphs
RNA-Seq data for ascites cells: TPM for tumor cells (TU), macrophages (TAM) and T cells (TAT) (Reinartz et al., 2016; Worzfeld et al., 2018).
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RNA-Seq data for omental metastases: TPM for tumor cells (TU), macrophages (TAM), mesothelial cells (MESO), adipocytes (ADI) and fibroblasts (CAF) (Sommerfeld et al., 2021).
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Proteomic data:
LC/MS-MS analysis (median LFQ) of TU, TAM and TAT from ascites (Worzfeld et al., 2018).
Secretome data:
LC/MS-MS analysis (median LFQ) of conditioned medium from ascites-derived TU, TAM and TAT (5-hr culture in protein-free medium) (Worzfeld et al., 2018).
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SOMAscan affinity proteomics data:
SOMAscan signals reflecting relative levels of protein mediators in ascites, plasma from ovarian cancer patients (OC-plasma) and patients with non-malignant gynecologic diseases (N-plasma) (Finkernagel et al., 2019).
Box pots show medians (horizontal line), upper and lower quartiles (box), range (whiskers) and outliers/fliers (diamonds).
Association of transcriptome and survival data:
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RFS associated with TCGA microarray data.
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KM-Plotter RNA microarray / relapse-free survival (RFS) data for HGSC (Gyorffy et al., 2012).
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PRECOG RNA-Seq / overall survival data for ovarian cancer (Gentles et al., 2015).
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SOMAscan ascites protein levels / RFS data for HGSC (Finkernagel et al., 2019).
TCGA, KMP: data split at best fitting quantile; PRECOG: data split at median.
A z-zcore of >1.96 or <-1.96 corresponds to a logrank p-value of <0.05.
Positive z-scores indicate a hazard ratio >1 (shorter survival); negative z-scores indicate a hazard ratio <1 (longer survival).